the human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation

Abstract objective knowledge about the cellular mechanisms involved in human squamous esophageal steady state after steady state and chronic injury is limited.
Our goal is to better understand these mechanisms by using a functional 3D approach.
The expression of designed proliferation, mitosis, and ancestral lineage markers was evaluated in 10 patients with normal squamous esophagus by immune fluorescence on the 3D epithelial whole stent.
Cells that expressed different levels of epithelial and ancestral markers were isolated using flow microscopy and characterized by qPCR and IF. Their self-
The potential for renewal was studied by colony formation cell test and in vitro organ culture model.
Results the basal layer of the nipple had the highest proliferation and filament division activity and decreased linearly to the tip of the nipple (p

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